Spinal neurons retrogradely labeled from the rostral ventromedial medulla, and spinal neurons labeled for GlyT2 and/or GAD67 mRNAs.

<p>Fluorescence micrographs showing spinal neurons (red) retrogradely labeled with fluorescent microspheres from the rostral ventromedial medulla (RVM) and spinal neurons (green) containing GlyT2 and/or GAD67 mRNA (glycinergic and/or GABAergic neurons) identified with FISH. Arrowheads indicate spinal neurons in the area around the central canal, which are retrogradely labeled from the RVM and are also labeled for GlyT2 mRNA in segment C5 (<b>A</b>) and for GlyT2 and/or GAD67 mRNAs in segments L5 (C) and L6 (<b>B and D</b>). Scale bar = 50 µm.