Transverse sections through the spinal cord at the brachial level showing the axonal tracts

eGFP+ axons are found in motor nerves, in the ventral commissure and in the sensory nerves, that is, corresponding to the tracts originating from the different locations where transgenic cells are detected. Immunodetection of BEN/SC1/DM-GRASP (red) reveals an enlarged ventral root on the electroporated side compared to the contralateral control side (underlined with dashed lines in (d)). (a,d) Images obtained with an epifluorescent microscope. Co-immunodetection of BEN/SC1/DM-GRASP (red) and P-H3 (blue) 72 h after electroporation with CyclinD1 reveals the presence of mitotic cells displaying an axon. (e-f) Single optical sections; (f) high magnification of the cell marked with an arrow in (e) – the GFP channel is off. Single optical sections showing eGFP and Islet1/2 (red) 72 h after CyclinD1 electroporation. The population of motor neurons is increased on the transgenic side. Single optical sections showing eGFP, Islet1/2 (red) and BrdU (blue). (j) A high magnification of (i). Seventy-two hours following forced expression of a tagged version of CyclinD2, cells expressing GFP (green) still express CyclinD2 visualized with an anti-Tag-V5 antibody (red). The TagV5 is clearly detected in the differentiating field. (k,l) Maximal projections.<p><b>Copyright information:</b></p><p>Taken from "Forcing neural progenitor cells to cycle is insufficient to alter cell-fate decision and timing of neuronal differentiation in the spinal cord"</p><p>http://www.neuraldevelopment.com/content/3/1/4</p><p>Neural Development 2008;3():4-4.</p><p>Published online 13 Feb 2008</p><p>PMCID:PMC2265710.</p><p>