SPR sensograms and fits for PKCε inhibiting VHHs.

<p>SPR sensograms and fits for a second-order Langmuir binding model of VHH E6 (A) and a first-order Langmuir binding model of VHH G8 (B). The VHH injection time was 3 min, followed by a dissociation time of 5 min. The surface was regenerated with an injection of 10 mM NaOH for 3 min, followed by a stabilization time of 5 min between each VHH injection. Five concentrations of each VHH were used, with the middle concentration injected twice as an internal control. The VHH concentrations (in µg/ml) are marked adjacent to each fit on the right hand side of the figure.