Saturation binding analysis of lamprey, skate, bichir, and human VDR to [³H]-1, 25D₃.

<p>Saturation binding profiles are shown for lamprey VDR (red), skate VDR (orange), bichir VDR (green), and human VDR (blue). Lysates were prepared from transfected Cos7 cells as described in Materials and Methods. Lysates were incubated with 0–1.6 nM [³H]-1, 25D₃ for 18 hours at 4°C. Unbound ligand was removed as described. Specific binding values were calculated by subtracting the average non-specific binding counts from the total binding counts. Hyperbolic one-site binding curves were fit using Prism 4. The reported dissociation constant (K_d) in the manuscript for each VDR is the average of three or four separate experiments ± SEM. The graphs shown here are specific binding data from a representative experiment.