The expression of IL-1β and TNF-α after exposure to light.

<p>(A–C) The protein expressions of IL-1β and TNF-α in the retina began to increase at 6 hours after exposure to light and peaked at 3 days. (D–E) The mRNA expression of IL-1β and TNF-α in the retina was increased, and peaked at 1 day after photic injury as detected by real-time PCR. (F–G) ELISA assay detected the upregulated secretion of IL-1β and TNF-α in the 661W/microglia co-culture supernatant. (H–I) Compared with untreated co-cultures, the group pretreated with neutralizing CX3CR1 antibody showed significantly decreased expression of IL-1β at 12 hours and 24 hours after exposure to light. For TNF-α, statistical analysis showed significantly reduced production in the treated cultures at 12 hours. No difference was found between treated and untreated co-cultures at 24 hours.