PC12 cells were treated with propofol (10, 20, 50 µmol/L) and 3-MA 20 mmol/L) during OGD treatment, and the cells were harvested 6 h later.

<p>(A) Immunoblotting LC3 in PC12 cells after OGD. Propofol at concentrations of 20 and 50 µmol/L and 3-MA at a concentration of 20 mmol/L effectively blocked the activation of autophagy, as evidenced by inhibiting the production of LC3-II. (B) The optical densities of the respective protein bands were analyzed using Sigma Scan Pro 5 and normalized to the loading control (GAPDH). The results are expressed as the mean ± SD from six independent experiments. Statistical comparisons were conducted using an ANOVA followed by the Tukey test. **<i>p</i> < 0.01 vs. control group; ^#<i>p</i> < 0.05 vs. OGD-treated group.