Quantification of infected cells by flow cytometry.

<p>Silenced and non-silenced HepG2 cells were infected by DENV-2 at MOI of 2. Result showed a marked reduction in percentage of infected cells by flow cytometry. This figure shows the percentage of DENV infected cells at different conditions. (a) Transfected non-infected HepG2 cells (0.0%) represent the negative control. (b) Transfected mock-infected HepG2 cells as a staining control (0.0%). (c) Scramble siRNA transfected infected HepG2 cells (45.7%) as a positive control. (d) GRP78 silenced infected HepG2 cells (8.5%). (e) CLTC silenced infected HepG2 cells (8.5%). (f) DNM2 silenced infected HepG2 cells (15.2%). (g) GRP78, CLTC, and DNM2 combined silenced infected HepG2 cells (4.7%). (h) Summarized the results of the flow cytometry experiments. Data is expressed as a percentage of infected cells compared with scramble siRNA transfected infected HepG2 cells which was defined as 100%. The percentages of the infected cells are 18.7%±0.7, 18.6%±2.6, 33.4%±7.9, and 10.3%±3.9 for GRP78, CLTC, DNM2, and combined silenced HepG2 cells respectively (One-way ANOVA with Dunnett's post-test, P<0.0001, Results are expressed as mean ± SD from a representative experiment performed in triplicate). Also, statistical analysis shows no significant difference between scramble siRNA transfected and non-transfected infected HepG2 cells (One-way ANOVA with Dunnett's post-test, P>0.05).