α-enolase in the conditioned medium of DENV-infected cells is not due to cell death.

<p>HepG2 cells were either mock-infected or infected with DENV (MOI = 1, 2 and 4) for 24 h. (A) Cell viability was measured by trypan blue exclusion assay. Graph shows mean ± SEM from 5 independent experiments. (B) Cell viability was assessed by MTT reduction assay. Graph shows mean ± SEM from 6 independent experiments. (C) Cell lysis was assessed by LDH release assay. Graph shows mean ± SEM from 5 independent experiments. Differences between samples from mock-infected and DENV-infected cells were assessed using repeated measures ANOVA test.