BDNF and TrkB do not interact with AT1R in RVLM.

<p>Illustrative gels or summary of fold changes in angiotensin receptor subtype 1 (AT1R) or subtype 2 (AT2R) mRNA (A,C) against sham-control detected by real-time PCR (A,C) or AT1R or AT2R protein detected by Western blot analysis (B,D) in samples collected from bilateral RVLM 180 min after microinjection of KA (0.5 nmol) or PBS into the left hippocampal CA3 subfield of animals that received pretreatment by application into the bilateral RVLM of distilled water or 0.1 M PBS (Veh), BDNF ASON or SON (100 pmol), TrkB-Fc (1.5 pmol) or TrkA-Fc (1.5 pmol). Values are mean ± SEM of triplicate analyses on individual samples obtained from 5–6 animals per experimental group. *<i>P</i><0.05 versus sham-control or PBS group in the post hoc Scheffé multiple-range test. Note that in (B,D), dividing lines are placed on the gel images to denote groupings of images from different parts of the same gel or from different gels.