<p>M: size marker (GeneRuler 50–1000 bp DNA; Fermentas); PC: positive control; Clinical samples are denoted with consecutive numbers. Samples considered to be positive are marked with “+” sign. CoV1: alphacoronavirus, CoV2: betacoronavirus; RSV: respiratory syncytial virus; IAV: influenza A virus; PIV: parainfluenza virus type 1, 2 or 3; BoV: bocavirus; AdV: adenovirus; IBV: influenza B virus; hMPV: human metapneumovirus; EV: enterovirus. Analysis was performed on 1.5% agarose gel.</p
1<p>: Numbers in parenthesis are percentage of total nasopharyngeal aspirates.</p>2<p>: Only one vir...
<p>RT-PCR analysis of clinical specimens tested negatively for all known pathogens (denoted as “−”) ...
A multiplex polymerase chain reaction (PCR) for identification of four viruses causing acute respira...
<p>All primer pairs (names on top of the figure) were used to amplify all virus stocks included in t...
<p>Inf A, B, C: Influenza A, B, C; H1N1: Influenza A H1N1; H1N1pdm09: Influenza A H1N1pdm 2009; H3N2...
In up to 40 percent of the acute respiratory tract infections no pathogen is identified. In chapter ...
Molecular tests have gained a solid position in clinical virology. Developments in molecular diagnos...
<p>Underlined and bold numbers indicate significant differences in paired values by McNemar's test:<...
Accurate detection of respiratory viruses is important to guide antiviral therapy, prevent nosocomi...
Viral shedding profile of infections caused by the novel influenza A (pH1N1) virus has not been exte...
<p>M: size marker (GeneRuler 50–1000 bp DNA; Fermentas); CoV_1: HCoV-NL63, CoV_2: HCoV-HKU1; RSV: re...
Detection of a broad number of respiratory viruses is not undertaken currently for the diagnosis of ...
The introduction of the polymerase chain reaction (PCR) into the diagnostic setting has provided unp...
We have developed a mask sampler for exhaled respiratory viruses. Among a group of 9 patients with c...
<p>For each pathogen, the number of patients in whom this pathogen was detected (including single an...
1<p>: Numbers in parenthesis are percentage of total nasopharyngeal aspirates.</p>2<p>: Only one vir...
<p>RT-PCR analysis of clinical specimens tested negatively for all known pathogens (denoted as “−”) ...
A multiplex polymerase chain reaction (PCR) for identification of four viruses causing acute respira...
<p>All primer pairs (names on top of the figure) were used to amplify all virus stocks included in t...
<p>Inf A, B, C: Influenza A, B, C; H1N1: Influenza A H1N1; H1N1pdm09: Influenza A H1N1pdm 2009; H3N2...
In up to 40 percent of the acute respiratory tract infections no pathogen is identified. In chapter ...
Molecular tests have gained a solid position in clinical virology. Developments in molecular diagnos...
<p>Underlined and bold numbers indicate significant differences in paired values by McNemar's test:<...
Accurate detection of respiratory viruses is important to guide antiviral therapy, prevent nosocomi...
Viral shedding profile of infections caused by the novel influenza A (pH1N1) virus has not been exte...
<p>M: size marker (GeneRuler 50–1000 bp DNA; Fermentas); CoV_1: HCoV-NL63, CoV_2: HCoV-HKU1; RSV: re...
Detection of a broad number of respiratory viruses is not undertaken currently for the diagnosis of ...
The introduction of the polymerase chain reaction (PCR) into the diagnostic setting has provided unp...
We have developed a mask sampler for exhaled respiratory viruses. Among a group of 9 patients with c...
<p>For each pathogen, the number of patients in whom this pathogen was detected (including single an...
1<p>: Numbers in parenthesis are percentage of total nasopharyngeal aspirates.</p>2<p>: Only one vir...
<p>RT-PCR analysis of clinical specimens tested negatively for all known pathogens (denoted as “−”) ...
A multiplex polymerase chain reaction (PCR) for identification of four viruses causing acute respira...