A multiplex polymerase chain reaction (PCR) for identification of four viruses causing acute respiratory diseases in human beings was developed. The analytical sensitivity of developed RT-PCR for identification of adenovirus, respiratory-syncytial virus, flu viruses types A and B, and actual subtypes of type A flu virus (seasonal and pandemic variants H1N1, seasonal H3N2, and viruses of bird flu that are pathogenic to human beings H5 and H7) was 1 × 103 genome equivalents per milliliter. Diagnostic sensitivity for flu virus type A and B, and also subtypes H1 (seasonal H1N1, pandemic variant of H1N1 of year 2009), H3, H5 was 1 × 103-104 viral particles per milliliter. The method developed has high specificity and does not have positive signa...
BACKGROUND: The current spread of pandemic influenza A(H1N1)v virus necessitates an intensified surv...
International audienceRapid and specific diagnosis of influenza A/B and respiratory syncytial (RSV) ...
Use of multiplex real-time reverse transcription polymerase chain reaction (RT-PCR) for the simultan...
The novel influenza A (H1N1) virus is now rapidly spreading across the world. Early detection is one...
Novel respiratory viruses have been identified as possible agents of upper and lower respiratory tra...
BACKGROUND: Influenza A viruses are medically important viral pathogens that cause significant morta...
INTRODUCTION AND METHODS: Two real time one-step RT-PCR assays were developed for simultaneous detec...
Objective: Acute respiratory infections (ARI), ranging from the common cold to life threatening Inf...
The current spread of pandemic influenza A(H1N1)v virus necessitates an intensified surveillance of ...
A real-time RT-PCR assay was standardized and evaluated for the detection of the recent pandemic 200...
Traditional viral diagnostics such as viral culture and various serological techniques tend to be s...
Influenza viruses A play an important role in the structure of the incidence of people with acute re...
WOS: 000345811400013PubMed ID: 25492660This study was conducted to investigate the respiratory virus...
BACKGROUND: Influenza A viruses aremedically impor-tant viral pathogens that cause significant morta...
Viruses are the major contributors to the morbidity and mortality of upper and lower acute respirato...
BACKGROUND: The current spread of pandemic influenza A(H1N1)v virus necessitates an intensified surv...
International audienceRapid and specific diagnosis of influenza A/B and respiratory syncytial (RSV) ...
Use of multiplex real-time reverse transcription polymerase chain reaction (RT-PCR) for the simultan...
The novel influenza A (H1N1) virus is now rapidly spreading across the world. Early detection is one...
Novel respiratory viruses have been identified as possible agents of upper and lower respiratory tra...
BACKGROUND: Influenza A viruses are medically important viral pathogens that cause significant morta...
INTRODUCTION AND METHODS: Two real time one-step RT-PCR assays were developed for simultaneous detec...
Objective: Acute respiratory infections (ARI), ranging from the common cold to life threatening Inf...
The current spread of pandemic influenza A(H1N1)v virus necessitates an intensified surveillance of ...
A real-time RT-PCR assay was standardized and evaluated for the detection of the recent pandemic 200...
Traditional viral diagnostics such as viral culture and various serological techniques tend to be s...
Influenza viruses A play an important role in the structure of the incidence of people with acute re...
WOS: 000345811400013PubMed ID: 25492660This study was conducted to investigate the respiratory virus...
BACKGROUND: Influenza A viruses aremedically impor-tant viral pathogens that cause significant morta...
Viruses are the major contributors to the morbidity and mortality of upper and lower acute respirato...
BACKGROUND: The current spread of pandemic influenza A(H1N1)v virus necessitates an intensified surv...
International audienceRapid and specific diagnosis of influenza A/B and respiratory syncytial (RSV) ...
Use of multiplex real-time reverse transcription polymerase chain reaction (RT-PCR) for the simultan...