<p>Parasites co-transfected with the pTREXL-mCherry::TcAAP3 and pTREXL-Bilbo1::eGFP (upper panels) or pTREXL-eGFP::TcAAP7 (lower panels) were analyzed by fluorescence microscopy, 24 h post-transfection. Differential interference contrast (DIC), DAPI, eGFP (Marker), mCherry (TcAAP3) and the merged image (Merge) are showed in the indicated columns. White bars in the left corner of the merged images represent 5 µm.</p
<p>Cells bearing plasmid-borne <i>P</i><sub>ω</sub> ω:<i>yfp</i> and <i>P</i><sub>δ</sub> δ:<i>cfp</...
<p>A. Western blot using anti-GFP antibody demonstrating production of GFP-fusion proteins of the co...
<div><p>(A–C) Fluorescence LM images of a vacuole containing eight living transgenic parasites expre...
<p>A) Parasites co-transfected with the pTREX-TcAAAP069:mCherry and pTREX-Bilbo1:eGFP were analyzed ...
<p>(A) IIF assays of TCLP 1::3XFLAG-transfected epimastigotes were probed with monoclonal anti-FLAG ...
<p>Epimastigotes were transiently transfected with the plasmids pTREX-TcDPM1-GFP (<b>A</b>), pTREX-T...
(A) Hepatoma cells were infected with transgenic parasites expressing the indicated proteins fused t...
<p>From left to right: Brightfield, GFP-fluorescence, DAPI- (fixed cells; top) or Hoechst 33258- (li...
<div><p>(A) Fluorescence LM image of a vacuole containing eight living transgenic parasites expressi...
<p>(A) Wide-field epifluorescence images of live parasites expressing PfVps35-YFP. Parasites are sho...
A, Immunofluorescence assay of Pf3D7VP2KO-VP1-3HAapt. DAPI stains the nuclei. Green, PfVP1-3HA. Red,...
<p>Parasites transformed with GFP- or HA-tagged PfGDV1 were stained with DAPI (DNA stain) and the in...
<p>GFP fluorescence images showing localization of PvTRAg36.6-GFP in trophozoite stages of transgeni...
<p>A) Poly-A (Cy-5-labeled); B) β-tubulin (Cy-3-labeled); C) PFR2 (Cy-3-labeled); D) Cruzipain (Cy-5...
<p>Immunofluorescence assays of <i>T. gondii ku80::hxgprt</i> parasites expressing C-terminal endoge...
<p>Cells bearing plasmid-borne <i>P</i><sub>ω</sub> ω:<i>yfp</i> and <i>P</i><sub>δ</sub> δ:<i>cfp</...
<p>A. Western blot using anti-GFP antibody demonstrating production of GFP-fusion proteins of the co...
<div><p>(A–C) Fluorescence LM images of a vacuole containing eight living transgenic parasites expre...
<p>A) Parasites co-transfected with the pTREX-TcAAAP069:mCherry and pTREX-Bilbo1:eGFP were analyzed ...
<p>(A) IIF assays of TCLP 1::3XFLAG-transfected epimastigotes were probed with monoclonal anti-FLAG ...
<p>Epimastigotes were transiently transfected with the plasmids pTREX-TcDPM1-GFP (<b>A</b>), pTREX-T...
(A) Hepatoma cells were infected with transgenic parasites expressing the indicated proteins fused t...
<p>From left to right: Brightfield, GFP-fluorescence, DAPI- (fixed cells; top) or Hoechst 33258- (li...
<div><p>(A) Fluorescence LM image of a vacuole containing eight living transgenic parasites expressi...
<p>(A) Wide-field epifluorescence images of live parasites expressing PfVps35-YFP. Parasites are sho...
A, Immunofluorescence assay of Pf3D7VP2KO-VP1-3HAapt. DAPI stains the nuclei. Green, PfVP1-3HA. Red,...
<p>Parasites transformed with GFP- or HA-tagged PfGDV1 were stained with DAPI (DNA stain) and the in...
<p>GFP fluorescence images showing localization of PvTRAg36.6-GFP in trophozoite stages of transgeni...
<p>A) Poly-A (Cy-5-labeled); B) β-tubulin (Cy-3-labeled); C) PFR2 (Cy-3-labeled); D) Cruzipain (Cy-5...
<p>Immunofluorescence assays of <i>T. gondii ku80::hxgprt</i> parasites expressing C-terminal endoge...
<p>Cells bearing plasmid-borne <i>P</i><sub>ω</sub> ω:<i>yfp</i> and <i>P</i><sub>δ</sub> δ:<i>cfp</...
<p>A. Western blot using anti-GFP antibody demonstrating production of GFP-fusion proteins of the co...
<div><p>(A–C) Fluorescence LM images of a vacuole containing eight living transgenic parasites expre...