<p>A) Parasites co-transfected with the pTREX-TcAAAP069:mCherry and pTREX-Bilbo1:eGFP were analyzed by fluorescence microscopy. Differential interference contrast (DIC), DAPI, TcBilbo (flagellar pocket marker), Tc069 and the merged image (Merge) are showed in the indicated columns. B) A similar analysis as in (A) was performed but using the acidocalcisomes and contractile vacuole marker aquaporin (TcAQP) instead of TcBilbo. Parasites co-transfected with the pTREX-TcAAAP069:eGFP and pTREX-TcAQP:mCherry were analyzed by fluorescence microscopy. White bars in the left corner of the merged images represent 5 μM.</p
(A) Hepatoma cells were infected with transgenic parasites expressing the indicated proteins fused t...
(A) Highly synchronous parasites expressing the 120 aa, 190 aa and NT FP2a reporters were sorbitol s...
<p>A-Confocal images showing the localization of ROP2-4 and proROP4 proteins (red) in control (YFP-n...
<p>Parasites co-transfected with the pTREXL-mCherry::TcAAP3 and pTREXL-Bilbo1::eGFP (upper panels) o...
<p>(A) IIF assays of TCLP 1::3XFLAG-transfected epimastigotes were probed with monoclonal anti-FLAG ...
<p>From left to right: Brightfield, GFP-fluorescence, DAPI- (fixed cells; top) or Hoechst 33258- (li...
<p>(A) Wide-field epifluorescence images of live parasites expressing PfVps35-YFP. Parasites are sho...
<div><p>(A–C) Fluorescence LM images of a vacuole containing eight living transgenic parasites expre...
<p>Epimastigotes were transiently transfected with the plasmids pTREX-TcDPM1-GFP (<b>A</b>), pTREX-T...
A, Immunofluorescence assay of Pf3D7VP2KO-VP1-3HAapt. DAPI stains the nuclei. Green, PfVP1-3HA. Red,...
<div><p>(A) Fluorescence LM image of a vacuole containing eight living transgenic parasites expressi...
<p>A) Poly-A (Cy-5-labeled); B) β-tubulin (Cy-3-labeled); C) PFR2 (Cy-3-labeled); D) Cruzipain (Cy-5...
<p><b>(A)</b> Immunofluorescence assay of epimastigotes from HATcRPE1, TcRPE1HA, and HATcRPE2 CL Bre...
<p>Immunofluorescence assays of <i>T. gondii ku80::hxgprt</i> parasites expressing C-terminal endoge...
<p>A) Cruzipain (Cy-5-labeled); B) β-tubulin (Cy-3-labeled); C) PFR2 (Cy-5-labeled); D) merged image...
(A) Hepatoma cells were infected with transgenic parasites expressing the indicated proteins fused t...
(A) Highly synchronous parasites expressing the 120 aa, 190 aa and NT FP2a reporters were sorbitol s...
<p>A-Confocal images showing the localization of ROP2-4 and proROP4 proteins (red) in control (YFP-n...
<p>Parasites co-transfected with the pTREXL-mCherry::TcAAP3 and pTREXL-Bilbo1::eGFP (upper panels) o...
<p>(A) IIF assays of TCLP 1::3XFLAG-transfected epimastigotes were probed with monoclonal anti-FLAG ...
<p>From left to right: Brightfield, GFP-fluorescence, DAPI- (fixed cells; top) or Hoechst 33258- (li...
<p>(A) Wide-field epifluorescence images of live parasites expressing PfVps35-YFP. Parasites are sho...
<div><p>(A–C) Fluorescence LM images of a vacuole containing eight living transgenic parasites expre...
<p>Epimastigotes were transiently transfected with the plasmids pTREX-TcDPM1-GFP (<b>A</b>), pTREX-T...
A, Immunofluorescence assay of Pf3D7VP2KO-VP1-3HAapt. DAPI stains the nuclei. Green, PfVP1-3HA. Red,...
<div><p>(A) Fluorescence LM image of a vacuole containing eight living transgenic parasites expressi...
<p>A) Poly-A (Cy-5-labeled); B) β-tubulin (Cy-3-labeled); C) PFR2 (Cy-3-labeled); D) Cruzipain (Cy-5...
<p><b>(A)</b> Immunofluorescence assay of epimastigotes from HATcRPE1, TcRPE1HA, and HATcRPE2 CL Bre...
<p>Immunofluorescence assays of <i>T. gondii ku80::hxgprt</i> parasites expressing C-terminal endoge...
<p>A) Cruzipain (Cy-5-labeled); B) β-tubulin (Cy-3-labeled); C) PFR2 (Cy-5-labeled); D) merged image...
(A) Hepatoma cells were infected with transgenic parasites expressing the indicated proteins fused t...
(A) Highly synchronous parasites expressing the 120 aa, 190 aa and NT FP2a reporters were sorbitol s...
<p>A-Confocal images showing the localization of ROP2-4 and proROP4 proteins (red) in control (YFP-n...