PPM1A overexpression induces cell cycle arrest.

<p>FACS analysis <i>(</i><b><i>A</i></b><i>)</i>, and BrdU labeling <i>(</i><b><i>B</i></b><i>)</i> of PC6-3 cells overexpressing PPM1A wt. Cells were treated as described in the “<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0032438#s2" target="_blank">Materials and Methods</a>” and analyzed for DNA content by propidium iodide (PI) staining <i>(</i><b><i>A</i></b><i>)</i> and for BrdU labeling by anti-BrdU antibodies as represented by the dot plot of DNA content (PI) against BrdU incorporation <i>(</i><b><i>B</i></b><i>)</i>. <i>(</i><b><i>C</i></b><i>)</i> FACS analysis of PC6-3 cells overexpressing PPM1A mutant. Cells were incubated with or without Tet, and then analyzed for DNA content by propidium iodide (PI) staining. The numbers represented in the tables indicate % of total cells in <i>(</i><b><i>A</i></b><i>)</i> and <i>(</i><b><i>C</i></b><i>)</i>, and % of total BrdU labeled cells in <i>(</i><b><i>B</i></b><i>)</i>. The experiments in <i>(</i><b><i>A</i></b><i>)</i>, <i>(</i><b><i>B</i></b><i>)</i> and <i>(</i><b><i>C</i></b><i>)</i> were performed at least three times.