PP6R3 partially enhances <i>CLN1</i> expression and cell cycle progression.

<p>Isogenic WT (MLY41) and quadruple <i>sap</i> (JRY40) mutant strains transformed with vector or plasmid expressing <i>PP6R3</i> were grown to exponential phase and elutriated to enrich for G₁ cells. Elutriated cells were collected, transferred to fresh SD-Ura media, and samples were taken over a 3 hours period for G₁ functions analysis. (A) Samples were analyzed for DNA content by flow cytometry as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0006331#pone-0006331-g004" target="_blank">Figure 4A</a>. The percentage of cells with 2N DNA content is shown in the upper right corner of each panel). (B) Table shows the percentage of budded cells (ND, not detectable). (C) <i>CLN1</i> and <i>ACT1</i> mRNA expression was assessed by northern blot. (D) Northern blot signals from experiment shown in Panel C for <i>CLN1</i> were quantified and normalized to the <i>ACT1</i> loading control signal. Results shown are the relative percentage of gene expression with maximal level of expression at 30 min for WT as 100%.