HIV-1 infection of MDM inhibits phagocytosis of antibody opsonised CS2 infected erythrocytes.

<p>(<b>A</b>). MDM from 11 donors were cultured for 5 days and infected with HIV-1_Ba-L (•) at a MOI of between 0.1 and 1.0 or mock-infected (○) then cultured for an additional 7 days. IE opsonised with 9% PPS were added at a 20∶1 ratio and phagocytosis was measured at 1 hour. Phagocytic index represents the number of ingested IE per 100 MDM. Statistical comparisons were performed in a pair wise manner using the Wilcoxon matched pairs test. (<b>B</b>). In 6 selected cultures analysed for phagocytosis, XTT assay was used to measure viability of MDM infected with HIV-1_Ba-L (•) or mock-infected (○) MDM (3 mg/mL XTT, 4 hr, absorbance was read at 450 nm using a reference wavelength of 650 nm). (<b>C</b>). Effect of IFNγ on HIV-1_Ba-L production by MDM. MDM were cultured in triplicate in 96-well plates for 5 days, infected with HIV-1_Ba-L for a further 5 days then primed with 100 ng/mL IFNγ where indicated. After 72 hrs media from triplicate wells was collected and analysed by micro RT assay. Comparisons between unprimed and primed MDM were made using the Wilcoxon matched pairs test and a significant difference (p&lt;.05) is indicated.