Experimental results.

<p>A. Application 1, demonstrating the uncoupling of [Ca²⁺]_i and membrane potential variations. Blue, control: F₀(t)/V₀(t); black, hyperpolarization without uncaging: F₁(t)/V₁(t); magenta, hyperpolarization with uncaging: F₁(t)/V₁(t). A₁ Membrane potential variations. A₂ Ca²⁺-induced fluorescence variations (F₀(t) and F₁(t)) are not significantly correlated. A₃ Same, but with uncaging. B. Application 2, demonstrating Ca²⁺ channel function restoration. B₁ Membrane potential variations measured and played back -- the three superimposed traces are identical. B₂ Fluorescence traces for control (blue F₀(t)) and without uncaging (black, F_2a(t)) -- these were not significantly correlated. B₃ Same, but with uncaging (magenta F_2b(t)). C Application 3: standardization of [Ca²⁺]_i variations across neurons. F₀(t) and V₀(t) were recorded in one cell while the uncaging (F₃(t)) was performed in another that was kept hyperpolarized (V₃(t)). The same parameters for the conductance model were used for C_1-4 and endogenous dynamics were reliably reproduced in all cases (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0028685#pone-0028685-t002" target="_blank">Table 2</a>).