PKA dynamics.

<p><b>A</b>, Model network pathway of PKA dynamics. R and C represent the regulatory subunit and catalytic subunit of PKA, respectively, where the amount of PKA activity is considered identical to C activity. <b>B</b>, <b>C</b>, PKA activity in response to a 90 min 5HT application (<b>B</b>) or 5×5 min 5HT application (<b>C</b>). Black line represents the amount of cAMP activity; blue line represents C, and red line RC. R is not shown, as it is identical to C. (Model adapted from <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1002324#pcbi.1002324-Pettigrew2" target="_blank">[70]</a>). <b>D</b>, PKC Apl II translocation in response to 5×5 min application of 5HT with 15 min washes in between and anisomycin present throughout from Farah et al. (2009) (bars) and modeling output (line). <b>E</b>, Modeling of S dynamics in response to experimental protocol from <b>D</b>. Black line represents the ratio of S_OFF and S_ON to total S, the red line the ratio of S_IN2 to total S, and the blue line represents ratio of S_PKA to total S.