Quantitative phosphorylation analysis of Akt, MEK, and ERK in human osteoblasts induced by INSL3.

<p>Quantitative analysis on Akt phosphorylation: <b>A</b>. ELISA at S473, <b>B</b>. densitometric analysis at S473 and <b>C</b>. densitometric analysis at T308. Quantitative analysis on MEK phosphorylation: <b>D</b>. ELISA and <b>E</b>. densitometric analysis. Quantitative analysis on ERK phosphorylation: <b>F</b>. ELISA and <b>G</b>. densitometric analysis. ELISA data are shown as the mean ± standard deviation (SD) of the mean of three different experiments performed in triplicate. Densitometric data are shown as the mean ± standard deviation (SD) of the mean of three different experiments. * P<0.05 vs control.