Correlation between the activation status of the PI3K/AKT pathway and DEG expression in NSCLC cell lines.

<p><b>(A)</b> Immunoblot analysis of phosphorylated AKT in NSCLC cell lines. AKT1 and actin were used to normalize AKT phosphorylation and protein loading, respectively. <b>(B)</b> The graph shows mRNA levels of up-regulated DEGs (GDF15, PTGES, S100P) in NSCLC cell lines. Statistical analysis was performed on 3 independent experiments conducted in triplicate and was performed by One-way ANOVA test for each gene relative to each cell line, p-values as indicated <b>(C)</b> The graph shows mRNA levels of three representative down-regulated DEGs (SGK1, IGFBP3, PEG10) in NSCLC cell lines. Bars are mean ± SD of a representative experiment. Statistical analysis was performed on 3 independent experiments conducted in triplicate and was performed by One-way ANOVA test for each cell line, p-values as indicated.