MAP4 overexpression increases the amount of α-tubulin and MT networks.

<p><b>A</b> and <b>B</b>, Western blots showed that the expression of MAP4 and gross quantity of α-tubulin in HeLa cells and CMs after Ad-MAP4 was elevated after transfection. <i>Graphs</i> represent the mean±SEM of the relative optical density signals for three separate experiments (<i>n</i> = 3). N - non-transfected cells. # <i>P</i><0.01 vs. N and Ad-GFP, Student's <i>t</i> test analysis. Cytosol GAPDH was chosen as the internal control. <b>C</b> and <b>D</b>, Immunofluorescent confocal micrographs of HeLa cells and CMs. Micrographs show that cells contain a larger amount of MAP4 (<i>FITC-green</i>) and more luxuriant MT network structure (<i>TRITC-red</i>) after Ad-MAP4 transfection compared with Con (Non-transfected cells). Scale bar, 10 µm.