HIV gp120 binding to vaginal hMR and its affinity constants.

<p>(a) HIV gp120 binding and its inhibition to immunopurified hMR from human vaginal proteins and Vk2/E6E7 proteins. Results are mean ± SE, n = 3. *Statistically significant (p<0.05) when compared with controls. ^#Statistically significant (p<0.05) when compared with HIV gp120 treated cells. Saturation Isotherm of HIV gp120 binding to hMR immunopurified from (b) human vaginal proteins and (c) Vk2/E6E7 proteins at concentrations indicated on the X axis. Affinity constants, Kd = 2.9±0.4 nM and Kd = 3.2±0.6 nM for human vaginal proteins and Vk2/E6E7 proteins respectively. Results are a representative of three independent experiments.