Effect of HIV gp120 on MMP production.

<p>(a) Effect of increasing concentrations of HIV gp120 on mRNA expression of MMP-9 and MMP-2 in Vk2/E6E7 cells. (b) Effect of increasing concentrations of HIV gp120 on MMP-9 and MMP-2 activity. (c) Representative zymograms of HIV gp120 treated Vk2/E6E7 cells (upper panel); lane 1: untreated cells, lane 2: 0.083 nM of HIV gp120, lane 3: 0.83 nM of HIV gp120, lane 4: 83 nM of HIV gp120. Lower panel represents effect of anti-hMR antibody on activity of MMP-9 and MMP-2; lane 1: Cells treated with HIV gp120 (83 nM), lane 2: HIVgp120 (83 nM) + isotype IgG, lane 3: HIV gp120 (83 nM) + anti-hMR IgG, lane 4: untreated cells. (d) Effect of anti-hMR antibody on expression of HIV gp120 induced MMP-9 mRNA. (e) Effect of anti-hMR antibody on MMP-9 activity. (a) and (d) values are mean ± SE of relative expression normalized to 18S rRNA from 3 independent experiments. (b) and (e) are mean ± SE of MMP activity expressed as fold change over unstimulated cells from 3 independent experiments. *Statistically significant (p<0.05) when compared with controls. ^#Statistically significant (p<0.05) when compared with HIV gp120 treated cells.