Lymphoaccumulation and T cell phenotype in MRL-<i>Fas^lpr</i> mice.

<p>(A) T cell accumulation. LN cells were obtained from MRL-<i>Fas^lpr</i> and control C57BL/6 mice at the indicated ages. Frequency and number (± SD) of CD4⁺, CD8⁺ and DN T cells were determined by flow cytometry after gating on the TCRβ⁺ cell population. (B) T cell phenotype. Expression of CD44, CD69, CD25 and CD62L by TCRβ⁺CD4⁺, TCRβ⁺CD8⁺, and TCRβ⁺DN T cells was assessed by flow cytometry in LNs of young (Y, 8 weeks of age) and older (O, 12 weeks of age) MRL-<i>Fas^lpr</i> mice. Numbers indicate percentage of positive cells (± SD). Data are representative of 3–10 independent experiments with 3–5 mice/group.