Flow cytometric analyses of T-cells.

<p>(A) MRL^lpr, MRL^lprIl18^+/tm, and MRL^lprIl18^tm/tm mice were bled and frequencies of blood cells were analysed using antibodies recognizing CD3 and B220. Presented are individual data from 3–23 evaluations and the respective means +/- SD. The three data sets were analysed by ANOVA with Bonferroni’s post test and revealed no statistically significant differences. (B) Single cell suspensions were prepared from kidneys of 19–29 week old MRL^lpr and MRL^lprIl18^tm/tm mice, and frequencies of CD3⁺⁺, CD4⁺, and CD8⁺ cells within the CD45⁺ population were determined. Presented are data of individual evaluations and the respective means +/- SD. The data sets (n = 9 and 13, respectively) were analysed by Student’s T-test and revealed no statistically significant differences.