Replication capacity of NYVAC-C-KC and NYVAC-C-KC-ΔB19R in different human cells.

<p><b>A) Single-step growth curve in HeLa cells.</b> Cells were infected with the indicated viruses at an MOI of 5 and harvested at 0, 6, 12, and 24 hours post-infection. Error bars are based on two separate infections. <b>B) Multi-step growth curves in human keratinocytes.</b> Cells were infected with the indicated viruses at an MOI of 0.01 and harvested at 3 or 72 hours post-infection, plus or minus 1000 Units/ml IFN. Error bars are based on two separate infections. <b>C) Multi-step growth curves in human dermal fibroblasts.</b> Cells were infected with the indicated viruses at an MOI of 0.01 and harvested at 3 or 72 hours post-infection, plus or minus 1000 Units/ml IFN. Error bars are based on two separate infections.