CCoV-II infection modulates the gene regulation of SIRT3 and SIRT4.

<p> (<b>A</b>) To perform Northern blot assay, RNA was extracted from mock-infected (lanes cc, control cells) and infected cells at the indicated times, electrophoresed and hybridized with a labelled probe as described under <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0027313#s2" target="_blank">Material and Methods</a>. β-actin was used as loading control. Blot is representative of three separate experiments. (<b>B</b>) Densitometric analysis of blots relative to SIRT3. The bars represent the mean ± SEM of the results from three separate experiments. Significant differences between CCoV-II-infected cells and control cells are indicated by probability <i>P</i>. **<i>P</i> <0.01, and ***<i>P</i> <0.001. (<b>C</b>) Densitometric analysis of blots relative to SIRT4. The bars represent the mean ± SEM of the results from three separate experiments. Significant differences between CCoV-II-infected cells and control cells are indicated by probability <i>P</i>. **<i>P</i> <0.01.