Effect of P2Y2-R knockdown (via shRNA) on PM wound repair in RLE and Type 1 AECs.

<p>(a) P2Y2R protein expression in P2Y2R-shRNA lentivirus infected RLE cells (shRNA) compared to those that were infected with non-targeting shRNA lentiviral particles (CTL) and wild type RLE cells (WT) (top). Beta-actin was used as a loading control (bottom). (b) P2Y2R null (shRNA) and P2Y2R positive RLE (control) were subjected to micropuncture injury in the presence or absence of ATP and % repair was calculated according to the formula described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0027469#s4" target="_blank">methods</a> section (n = 21 cells/group). (c) P2Y2R null (gridded arrows) and P2Y2R positive (white arrow) type 1 AECs were subjected to micropuncture injury and % repair was calculated according to the formula described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0027469#s4" target="_blank">methods</a> section (n = 17 cells/ group) (* indicates p<0.05). Data are presented as means ± one standard error of the mean.