Effect of P2Y2-R silencing (via siRNA) on PM wound repair in A549 cells.

<p>(a) P2Y2R protein expression in P2Y2R-targeted siRNA transfected A549 cells (siRNA) compared to those transfected with scrambled control (CTL) and wild-type A549 (WT) (top). Beta-actin was used as a loading control (bottom). Western blots shown are results of four separate experiments (Exp1-4) (b) A549 cells transfected with P2Y2R-targeted siRNA (siRNA) or non-targeting scrambled control siRNA (control) were subjected to micropuncture injury. % repair was calculated according to the formula described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0027469#s4" target="_blank">methods</a> section (n = 17 cells/ group) (* indicates p<0.05). Data are presented as means ± one standard error of the mean.