Phylogenetic tree constructed using a maximum-likelihood method from the NFLG sequences of 64 patient samples and 37 HIV-1 reference sequences from the Los Alamos HIV-1 database representing 11 genetic subtypes.

<p>Annotation of samples is as follows: symbol-green circle (subtype B), symbol-blue square (subtype C), symbol-red triangle (subclade F1), symbol-brown square-M indicates heterosexual male, symbol-brown square-F indicates heterosexual female, and symbol-black square indicates homosexual male. The approximate likelihood ratio test (aLRT) values of≥90% are indicated. The scale bar represents 0.05 nucleotide substitutions per site.