Viral proteins and RNA in cell lysates and extracellular particles after DDX6 knockdown.

<p>HT1080 cells were transfected twice with 60 nM control siRNA or DDX6 siRNA. 1^st siRNA transfection was done at time 0. 2^nd siRNA transfection was performed at 24 h, followed by the infection with foamy virus at 48 h (i.e. 24 h after 2^nd siRNA transfection). Cells and culture supernatants were harvested at 45 h after infection. (A) Immunoblot analyses of Gag, Pol, DDX6, and GAPDH in infected cell lysates. (B) Immunoblot analyses of Gag and Pol in extracellular particles. (C) Relative quantities for viral infectivity, Gag and Pol proteins, and viral RNA in extracellular particles obtained from transfection with DDX6 siRNA compared to that with control siRNA. Means and standard errors were results for each analysis from at least four independent experiments.