Egr1 is transiently induced in ECV304 cells in response to EGF treatment.

<p>(<b>A</b>) ECV304 cells were treated for 30, 60 and 120 minutes with 10 ng/ml EGF. Lysates were immunoblotted with polyclonal antibody to EGR1 and, after stripping, with anti tubulin antibody to assess equal loading. (<b>B</b>) ECV304 cells were treated for 60 minutes with 10 ng/ml EGF in the absence or in the presence of 20 µM cycloheximide. Lysates were immunoblotted with polyclonal antibody to EGR1 and, after stripping, with anti tubulin antibody to assess equal loading. (<b>C</b>) ECV304 cells were treated for 60 minutes with 10 ng/ml EGF in the absence or in the presence of UO126 or wortmannin. Lysates were immunoblotted with polyclonal antibody to EGR1, phospho-ERK 1/2, ERK 1/2, phospho-AKT and AKT. All experiments are representative of three independent.