<p><b><i>A</i></b>) Following TNBS administration, LPM were harvested and stimulated with LPS (1 ug/mL) <i>ex vivo</i> (24 hours) and TNF-α secretion was measured (N = 8). Data are presented as % Non-Inflamed Non-treated control ± SEM. * Statistical difference (p<0.05), # statistical difference (p<0.001). B) LITAF message levels are increased in LPM harvested from inflamed colonic tissue following LPS (1 ug/mL) stimulation (1 hour) compared to LPM harvested from non-inflamed colonic tissue (N = 7 for Non-treated, N = 8 LPS treated). Data were normalized to β-Actin message and presented as % Non-Inflamed Non-treated group ± SEM. * Statistical difference (p<0.05).</p
Icited macrophages (MΦ) (n = 3) 24 hrs after exposure to LPS. The IC50 threshold (the concentration ...
Mφ (n = 6) were pre-treated with either 25(OH)D3 (100 nM and 500 nM) or 1.25(OH)2D3 (0.05 nM and 10 ...
<p><b>A</b>. IL-6 release was determined by ELISA in culture supernatants harvested 3 h, 6 h and 24 ...
<p><b><i>A</i></b>) Secreted TNF-α from LPM harvested from inflamed colonic tissue are reduced when ...
<p>TNF-α message levels were measured in colonic tissue following TNBS administration (0.3 mg) for 2...
<p><b><i>A</i></b>) Western blot of LITAF levels and GAPDH levels in freshly harvested LPM from Non-...
<p><b><i>A</i></b>) Western blot of LITAF and Actin confirming the knockout of LITAF protein in peri...
Dysregulation of TNF-a in lamina propria macrophages (LPM) is a feature of inflammatory bowel diseas...
Ction, mice were challenged i.p. with a lethal dose of 0.5 mg LPS then euthanized 2 hrs. later for m...
<p><b><i>A</i></b>) Representative histological images of colonic inflammation: Untreated Control; T...
<p>BM and SCM were activated with LPS to measure the difference in their pro-inflammatory molecules ...
<p>(A) Comparison of basal levels of <i>TNFA</i> mRNA in granulocytes among healthy controls, Group ...
Treatment with i.p. (panel A) or oral (panel C) LMP-420 at the indicated doses. LMP-420 doses of 5 m...
<p>Inflammation was induced by TNBS (1.5 mg, in 50% ethanol solution) and animals were monitored for...
Icited macrophages (MΦ) (n = 3) 24 hrs after exposure to LPS. The IC50 threshold (the concentration ...
Icited macrophages (MΦ) (n = 3) 24 hrs after exposure to LPS. The IC50 threshold (the concentration ...
Mφ (n = 6) were pre-treated with either 25(OH)D3 (100 nM and 500 nM) or 1.25(OH)2D3 (0.05 nM and 10 ...
<p><b>A</b>. IL-6 release was determined by ELISA in culture supernatants harvested 3 h, 6 h and 24 ...
<p><b><i>A</i></b>) Secreted TNF-α from LPM harvested from inflamed colonic tissue are reduced when ...
<p>TNF-α message levels were measured in colonic tissue following TNBS administration (0.3 mg) for 2...
<p><b><i>A</i></b>) Western blot of LITAF levels and GAPDH levels in freshly harvested LPM from Non-...
<p><b><i>A</i></b>) Western blot of LITAF and Actin confirming the knockout of LITAF protein in peri...
Dysregulation of TNF-a in lamina propria macrophages (LPM) is a feature of inflammatory bowel diseas...
Ction, mice were challenged i.p. with a lethal dose of 0.5 mg LPS then euthanized 2 hrs. later for m...
<p><b><i>A</i></b>) Representative histological images of colonic inflammation: Untreated Control; T...
<p>BM and SCM were activated with LPS to measure the difference in their pro-inflammatory molecules ...
<p>(A) Comparison of basal levels of <i>TNFA</i> mRNA in granulocytes among healthy controls, Group ...
Treatment with i.p. (panel A) or oral (panel C) LMP-420 at the indicated doses. LMP-420 doses of 5 m...
<p>Inflammation was induced by TNBS (1.5 mg, in 50% ethanol solution) and animals were monitored for...
Icited macrophages (MΦ) (n = 3) 24 hrs after exposure to LPS. The IC50 threshold (the concentration ...
Icited macrophages (MΦ) (n = 3) 24 hrs after exposure to LPS. The IC50 threshold (the concentration ...
Mφ (n = 6) were pre-treated with either 25(OH)D3 (100 nM and 500 nM) or 1.25(OH)2D3 (0.05 nM and 10 ...
<p><b>A</b>. IL-6 release was determined by ELISA in culture supernatants harvested 3 h, 6 h and 24 ...