Rapamycin experienced CTLs have increased proliferation and maintain higher CD62L expression during memory differentiation.

<p>Experimental setting was the same as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0025177#pone-0025177-g002" target="_blank">Figure 2</a>. Activated CTLs were harvested at day 3 after stimulation in vitro, and were transferred into naïve B6 mice at 10⁶/mouse. Blood samples were drawn at day 5, 15 and 30 after transfer. (A) Numbers of OT1 cells in spleen 5 days after transfer. Every group was compared to control group (rapamycin 0). (B) Representative CD62L expression in OT1 cells in blood at day 5 after transfer. (C) Comparison of CD62L expression in OT1 cells during memory differentiation. “In vitro” represents samples harvested at day 3 after stimulation in vitro. (D) In a separated experiment, rapamycin+3-SI programmed and control CTLs under 3-SI stimulation were harvested at 72 hrs, and labeled with CFSE before transferred into B6 mice. CFSE dilution was examined in OT1 cells from spleens at days 1 and 2 after transfer. Experiments are representatives of at least three independent experiments with similar results (A–C) or one experiment (D). Each value represents the mean plus the SD of 5 mice per group in C.