Altered temporal mRNA expression of clock genes in USP2^+/+ and USP2^{−<b>/</b>−} SCN and liver.

<p><i>Per1, Per2, Cry1, Rev-erbα</i>, <i>Bmal1</i>, and <i>Dbp</i> was measured by RT-qPCR and normalized to <i>RNA polymerase II</i> expression in SCN and liver isolated every 4 hours from USP2^+/+ (solid line) and USP2^−/− (dashed line) housed in a 12-hour LD cycle. The data was analyzed using the ΔCT method and peak mRNA expression was standardized at 100%. Data points are mean <u>±</u>SEM (n = 3 animals for each genotype). Two way ANOVA shows significance differences in the rhythms between wildtype and USP2^−/− mice (asterisks) for Per1 and Cry1 in SCN as well as BMAL1 and Dbp in liver (*p<0.05, ***p<0.001, two-way ANOVA).