Effects of miR-143 on PTGS2 expression in AECs and AMCs.

<p>A, qRT-PCR analysis shows that 50 nM of miR-143 mimic transfection significantly increases miR-143 expression, while transfection with 200 nM of miR-143 inhibitor has no effect in AECs. In AMCs, miR-143 mimic (50 nM) and hairpin inhibitor (200 nM) transfections significantly increased and decreased the expression of miR-143, respectively. miR-143 and miR-145 expression levels were normalized with 5S rRNA expression. B, Immunoblot analysis of PTGS2 expression in AECs and AMCs transfected with mimic negative control (lane 1), mimic-miR-143 (lane 2), hairpin inhibitor negative control (lane 3), and inhibitor-miR-143 (lane 4). In AECs, PTGS2 protein expression was 1.5-fold decreased in transfection with miR-143 mimic (p = 0.014), but no changes in transfection with miR-143 inhibitor. In AMC, miR-143 mimic and hairpin inhibitor transfections 1.8-fold increased and 1.3-fold decreased the expression of PTGS2 protein (p = 0.014 for each), respectively. PTGS2 protein level was normalized to that of β-actin. C, qRT-PCR analysis of PTGS2 mRNA in transfected AEC and AMC shows there are no significant differences. The PTGS2 mRNA expression was normalized on the content of RPLPO. n = 4 for each experiments; the graphs show means and SE. *, <i>p</i><0.05.