Overexpression of miR-424 inhibits the cell proliferation in HepG2.

<p>(A) qRT-PCR analysis of miR-424 expression after the transfection of miR-424 mimics, inhibitors or scramble or no transfection. (B) The CCK8 assay used to evalute the proliferation of HepG2 cells after transfection with the miR-424 mimics, inhibitors or scramble or no transfection. (C) (C) miR-424 inhibited Ki-67 mRNA expression. HepG2 cells were transfected with miR-424 mimics, inhibitors or scramble or no transfection. Ki-67 mRNA levels were detected by real-time PCR. The expression of Ki-67 was normalized to GAPDH. (D) miR-424 inhibited Ki-67 protein expression. HepG2 cells were transfected with miR-424 mimics, inhibitors or scramble or no transfection. Ki-67 levels were detected by western blot. GAPDH was also detected as a loading control. Values are presented as the mean ± SD. Compared with control, *p<0.05, ** p<0.01, and ***p<0.001.