TIG3 induces apoptosis.

<p><b>A</b> SCC-13 cells were infected with 10 MOI of EV or TIG3-encoding virus and after 24 h lysates were prepared for detection of apoptosis markers. The bracket indicates high molecular weight crosslinked TIG3 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0023230#pone.0023230-Sturniolo1" target="_blank">[10]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0023230#pone.0023230-Sturniolo2" target="_blank">[11]</a>. Molecular weights are indicated to the left of the blot in kDa. <b>B</b> At 24 h post-infection SCC-13 cells were fixed and stained with anti-cleaved PARP (red). TIG3 increases cleaved PARP staining. The percentage of cleaved PARP positive cells is presented in each panel (mean ± SD). The arrows indicate cleaved PARP-positive cells. Similar results were observed in each of three experiments.