Pericentrosomal organelle accumulation in TIG3-positive cells.

<p><b>A</b> SCC-13 cells were infected by 10 MOI tAd5-EV or tAd5-TIG3 and after 24 h fixed and stained to detect TIG3 (green) and various organelle markers (red). The markers include GM130 (cis-Golgi), M6PR (mannose-6-phosphate receptor, trans-Golgi and late endosome), rab11 (recycling endosome), calnexin (ER), and CHC (clathrin heavy chain, intracellular transport vesicle). White arrows indicate pericentrosomal localization of TIG3. Nuclei are stained blue. For GM130, M6PR, and rab11, all panels are red/green/blue merged images. The EV and TIG3 pictures of calnexin staining are red/green/blue merged images. The inserts in the TIG3/calnexin picture are single-color images. For the CHC stain, only the red (CHC) image is shown. Bars = 10 µm. <b>B</b> TIG3 impact on subcellular organelle distribution. To measure organelle spread, the microscope was focused on the z-plane displaying the maximal organelle spread and the area covered by the organelle was monitored using ImageJ Software and presented as organelle area in µm². The values are mean ± SEM (n = 30 fields) including measurement of a minimum of thirty cells per treatment group. Paired Student's t-test analysis identifies the means as significantly different (p<0.001).