Effect of MPL mutations on rotation angle along the membrane axis (ie, the azimuthal angle).

<p>Left panel: depiction of the azimuthal angle, defined as the torsion between 4 C_α atoms (red dots) of the MPL intracellular domain. The gold balls represent the phosphorus atoms of the membrane bilayer. Right panel: distribution of azimuthal angle in all simulations; the average values are indicated next to the mutant name. The distribution is calculated from the last 100 ns trajectory for each simulation with a sampling frequency of 100 ps (i.e., 1,000 data points for each mutant).