EMT of ARPE-19 cells induced by TGF-β1.

<p>ARPE-19 cells were grown on glass coverslips for 24 hr, starved for 24 hr, and then incubated for 24 hr, 48 hr, 72 hr with 10 ng/ml TGF-β1. Compared with control cells (A, E, I, M, Q), stimulated cells displayed an altered mesenchymal morphology by phase-contrast microscopy (B, C, D), with decreasing expression of E-cadherin (F, G, H) and ZO-1 (J, K, L) and an increasing expression of fibronectin (N, O, P) and α-SMA (R, S, T) by immunofluorescence microscopy. The green signal represents the staining of corresponding protein, and the red signal represents the nuclei staining by DAPI. Original magnifications 100× (A–D); 400× (E–T).