Diminished Dmc1/Rad51 focus formation in <i>Arf</i>-deficient spermatocytes.

<p>(A, B) Surface spread spermatocytes from three month old WT (left panels) and <i>Arf</i>-null (right panels) mice were immunostained for SCP3 (A, B; green) and Dmc1 (A; red) or Rad51 (B; red). Images were captured with the same exposure time using a Marianas spinning disc confocal microscope. (C, D) Histograms showing the distribution of the number of Dmc1 (C) and Rad51 (D) foci found in wild-type (blue bars) and <i>Arf</i>-null (red bars) spermatocytes. Foci were enumerated from one hundred zygotene spermatocytes immunostained for SYCP3 and either Dmc1 (C) or Rad51 (D) using Slidebook 5.0 SDC software. Actual values of foci per cell are plotted within bin ranges to display the distribution of frequencies. (E, F) Histograms showing the distribution of average intensities of Dmc1 (C) and Rad51 (D) foci found in wild-type (blue bars) and <i>Arf</i>-null (red bars) spermatocytes analyzed in panels C and D.