RNF8-dependent establishment of H3K27ac and its inhibition by H2AK119ub.

<p>(<b>A-D</b>) Immunostaining of SYCP3 and H3K27ac on meiotic chromosome spreads. Dotted circles: sex chromosomes. Scale bar: 10 μm. Representative images are shown for at least 30 spermatocytes from each substage, from at least 3 independent mice per mouse model. The intensity of immunostaining is quantified by densitometry across the indicated path (‘ to”) and plotted in a relative intensity range of 0–1, which is normalized among all pictures in <b>A-D</b>. (<b>E</b>) Model of the regulation of H3K27ac. (<b>F</b>) Immunostaining of SYCP3 and H3K27ac on wild-type and <i>Rnf8</i>-KO round spermatids using slides that preserve the relative nuclear organization of spermatogenic cells. Dotted circles: post-meiotic sex chromatin. Scale bar: 10 μm. Representative images are shown for at least 30 round spermatids from at least 3 independent mice.