Meiotic pairing configuration, C-banding and GISH patterns of wheat- <i>P. huashanica</i> progeny lines.

<p><b>a</b> Meiotic metaphase I pairing in the PHW-SA×J-11 F₁ hybrid: 2n = 49 with 13 I+15 II (ring)+3 II (rod); <b>b</b>, <b>c</b> C-banding and GISH patterns of PW11 (2n = 43), which contained 42 wheat chromosomes and one 3Ns chromosome of <i>P. huashanica</i> (yellow-greenish); <b>d</b>, <b>e</b> Meiotic metaphase I cell of PW11-2 (2n = 44) and PW11-8 (2n = 42) with 2 I+18 II (ring)+3 II (rod) and 20 II (ring)+1 II (rod), respectively; <b>f</b> C-banding pattern of PW11-2 (2n = 44), which included 42 wheat chromosomes and a pair of 3Ns chromosomes of <i>P. huashanica</i>; <b>g</b> C-banding pattern of PW11-5 (2n = 42), where two wheat 1B chromosomes were replaced by a pair of 3Ns chromosomes of <i>P. huashanica</i>; <b>h</b> C-banding pattern of PW11-8 (2n = 42), which contained 40 wheat chromosomes, one pair of wheat- <i>P. huashanica</i> terminally translocated chromosomes (arrows indicate a pair of 3BL-3NsS translocated chromosomes); <b>i</b>–<b>k</b> GISH using <i>P. huashanica</i> total genomic DNA labeled with digoxigenin-11- dUTP as probe and Chinese Spring genomic DNA as block (The <i>P. huashanica</i> chromatin fluoresced yellow-greenish, and the wheat chromatin fluoresced red). <b>i</b> GISH pattern of PW11-2 (2n = 44); <b>j</b> GISH pattern of PW11-5 (2n = 42); <b>k</b> GISH pattern of PW11-8 (2n = 42) (arrows indicate 3BL-3NsS translocated chromosomes); <b>l</b> Magnified picture of the translocated chromosome, from left to right: C-banded wheat chromosome 3B, C-banding of <i>P. huashanica</i> chromosome 3Ns, C-banding and GISH image of the 3BL-3NsS translocated chromosome.