Dual-color FISH/GISH identification of the homoeologous group of partially translocated wheat chromosomes.

<p><b>A</b>_<b>1</b><b>-R</b>_<b>1</b>: GISH patterns of wheat-<i>A</i>. <i>cristatum</i> translocation lines. Total genomic DNA of <i>A</i>. <i>cristatum</i> was labelled with digoxigenin-11-dUTP and visualized with red fluorescence. Chromosomes were counterstained with DAPI and visualized with blue fluorescence. <b>A</b>_<b>2</b><b>-R</b>_<b>2</b>: FISH patterns of wheat-<i>A</i>. <i>cristatum</i> translocation lines. <b>A</b>_<b>2</b><b>-N</b>_<b>2</b>, <b>P</b>_<b>2</b><b>-R</b>_<b>2</b>: The repetitive sequence clone pHvG39 was labelled with biotin-16-dUTP and visualized with green fluorescence; <b>O</b>_<b>2</b>: The repetitive sequence clone pSc119.2 was labelled with biotin-16-dUTP and visualized with green fluorescence. The repetitive sequence clone pAs1 was labelled with digoxigenin-11-dUTP and visualized with red fluorescence. Chromosomes were counterstained with DAPI and visualized with blue fluorescence. Arrows point to the translocated chromosomes. <b>A.</b> 2<b>P</b>-23; <b>B.</b> 2<b>P</b>-35; <b>C.</b> 2<b>P</b>-40; <b>D.</b> 2<b>P</b>-43; <b>E.</b> 2<b>P</b>-48; <b>F.</b> 2<b>P</b>-55; <b>G.</b> 2<b>P</b>-80; <b>H.</b> 2<b>P</b>-116; <b>I.</b> 2<b>P</b>-122; <b>J.</b> 2<b>P</b>-167; <b>K.</b> 2<b>P</b>-173; <b>L.</b> 2<b>P</b>-187; <b>M.</b> 2<b>P</b>-190; <b>N.</b> 2<b>P</b>-205; <b>O.</b> 2<b>P</b>-213; <b>P.</b> 2<b>P</b>-269; <b>Q.</b> 2<b>P</b>-355; <b>R.</b> 2<b>P</b>-367.