Nedd9<i>^−/−</i> mammary tumors have increased E-cadherin at cell junctions.

<p><b>A</b>, <b>B</b>. Immunohistochemical evaluation of MMTV-PyVT;<i>Nedd9^−/−</i> and MMTV-PyVT <i>Nedd9^+/+</i> mammary tumors for E-cadherin (<b>A</b>) and β-catenin (<b>B</b>) protein expression and localization. Insets (thick lines) are magnifications of indicated areas (thin lines). Three representative independently arising tumors are shown for each genotype. Scale bar, 100 µm. <b>C</b>. Western analysis of total lysates isolated from 9 independent MMTV-PyVT;<i>Nedd9^−/−</i> and MMTV-PyVT <i>Nedd9^+/+</i> mammary tumors and from MCF7 cells were probed for content of E-cadherin and β-catenin. β-actin served as loading control.