Expression of E-cadherin and sLe^x throughout the metastatic progression of MARY-X and canine mammary carcinoma.

<p>(A, B) Shows a heterogeneous population of cellular expression in both (A) MARY-X primary tumors and (B) canine primary tumors (carcinosarcoma and solid carcinoma) displaying a dominant population of cells with membrane-positioned E-cadherin (green) and smaller nests sLe^x (red) expressing cells (cytoplasmic or membrane-positioned). (A, B; middle panels) Magnified image shows the sLe^x found either cytoplasmic- (long arrows) or membrane-positioned (blunt arrows). Mary-X lung metastasis (MARY-X metastasis) displays a dominant population of cells expressing only E-cadherin (green), and a minority population of cells co-expressing cytoplasmic-positioned sLe^x (red) (long arrows). (B, C) Canine lung metastasis (Canine metastasis) displays a dominant population of cells expressing only E-cadherin (green), and minority populations of cells either co-expressing cytoplasmic-positioned sLe^x (red) (long arrows) or expressing only membrane-positioned sLe^x (red) (blunt arrows).