<i>Tspyl2</i> mutant mice have normal proliferation of neuronal precursor cells.

<p>(<b>A</b>) The expression pattern of <i>Tspyl2</i> as revealed by northern blot. Equal loading of RNA was visualized by ethidium bromide staining. (<b>B</b>) qPCR analysis of <i>Tspyl2</i> transcript in cerebral cortex, hippocampus (Hippo), testis, ovary and thymus in adult mice (n = 3–5). The relative expression of each tissue is expressed as the fold difference when compared with the thymus after normalization with <i>Hprt</i>. (<b>C</b>) Gross morphology of the brain dissected from wildtype (+/Y) and <i>Tspyl2</i>^m/Y (m/Y) adult mice after perfusion fixation. (<b>D</b>) BrdU staining in dentate gyrus of hippocampus and lateral ventricle of 2 months old adult mice. Nuclei were stained by DAPI. Scale bar: 50 µm. The number of BrdU-positive cells was counted and shown on the right. Error bar: SEM.