CAR dependent <i>Sult1e1</i> gene induction.

<p>(A) Wild type and <i>Car</i> null mice were given DAS (80 mg/100 g body weight) or DADS (8 mg/100 g body weight) by gastric gavage. 24 hrs after chemical administration, <i>Sult1e1</i> and <i>Car</i> gene expression was analyzed by quantitative PCR as described in the methods section. The relative mRNA amount of each gene was calculated so that wild type vehicle treated samples have one unit of expression. (B) Left panel; Liver cytosol extracts from the same mice used in (A) were prepared and SULT1E1 protein content was analyzed by western blotting. Recombinant mouse SULT1E1 protein was used as positive control and indicated with R, while V represents vehicle treatment. Anti Hsp70 western blotting is shown as a loading control. Right panel; Estrogen sulfotransferase activities were measured for liver extracts from the same mice used in (A). n.d. stands for no activity detected. (C) Liver nuclear extracts from the same mice used in (A) were prepared and CAR protein content was analyzed by western blotting. Anti Laminβ western blotting is shown as a loading control.