A: CAR expression in the liver and the adrenal glands.

<p>Total RNAs were extracted from liver biopsies or total adrenal glands and subjected to reverse transcription and PCR amplification. The PCR products were separated by electrophoresis. B and C: Mice were injected systemically with 10⁹ PFU of either AdHwt (n = 7) or AdH[GA]24 (n = 7) or PBS (n = 4). Forty-eight hours later, the animals were culled and the livers and adrenal glands were collected. B: Whole liver were stained for β-galactosidase expression. C: Total RNA were extracted from the adrenal glands (two glands from the same animal were pooled), reversed-transcribed and subjected to quantitative PCR to detect LacZ expression or 18S RNA. The ratio LacZ/18S of adrenal glands collected from animals injected with AdHwt was used as 100%. The data presented are means+SEM. Statistical test: ANOVA (Prism, Graph-Pad softwares).